Jagiellonian University 3 articles published in JoVE Neuroscience A Naturalistic Setup for Presenting Real People and Live Actions in Experimental Psychology and Cognitive Neuroscience Studies Tuğçe Nur Pekçetin1, Şeyda Evsen2, Serkan Pekçetin3, Cengiz Acarturk4, Burcu A. Urgen5 1Department of Cognitive Science, Middle East Technical University, 2Interdisciplinary Neuroscience Graduate Program, Bilkent University, 3Department of Computer Engineering, Middle East Technical University, 4Department of Cognitive Science, Jagiellonian University, 5Department of Psychology, Interdisciplinary Neuroscience Graduate Program, National Magnetic Resonance Research Center (UMRAM), Aysel Sabuncu Brain Research Center, Bilkent University This study presents a naturalistic experimental setup that allows researchers to present real-time action stimuli, obtain response time and mouse tracking data while participants respond after each stimulus display, and change actors between experimental conditions with a unique system including a special transparent organic light-emitting diode (OLED) screen and light manipulation. Immunology and Infection Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines Aneta Babula*1,2, Izabela Siemińska*1,3, Monika Baj-Krzyworzeka1 1Department of Clinical Immunology, Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences, Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them. Medicine Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings Dariusz Bazaliński1,2, Paweł Więch2, Paulina Szymańska3, Marek Muster2, Maria Kózka4 1Father B. Markiewicz Podkarpackie Specialist Oncology Centre, Specialist Hospital in Brzozów, 2Institute of Health Sciences, College of Medical Sciences, University of Rzeszów, 3General Surgery Clinic, Specialist Hospital in Radom, 4Department of Clinical Nursing, Faculty of Health Sciences, Collegium Medicum, Jagiellonian University Maggots of Lucilia sericata were used in the debridement of deep pressure sores in outpatient settings for the treatment of pressure sores involving the complete skin thickness. The technique presented in the article does not pose risks for the patient.