Federal Rural University of Rio de Janeiro 1 article published in JoVE Immunology and Infection Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies Jéssica Fiorotti1, Patrícia S. Gôlo1,2, Allan Felipe Marciano1, Mariana G. Camargo1, Isabele C. Angelo1,3, Vânia R. E. P. Bittencourt1,2 1Post-Graduate Program in Veterinary Sciences, Federal Rural University of Rio de Janeiro, 2Department of Animal Parasitology, Federal Rural University of Rio de Janeiro, 3Department of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro Analysis of tick hemolymph represents an important source of information on how some pathogens cause disease and how ticks immunologically respond to this infection. The present study demonstrates how to inoculate fungal propagules and collect hemolymph from Rhipicephalus microplus engorged females.