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Protein Crystallization for X-ray Crystallography

Article DOI: 10.3791/2285-v • 09:27 min • January 16th, 2011
January 16th, 2011 •
1, 1
1Molecular Biochemistry and Biophysics, Yale University

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The 3-D structure of a molecule provides a unique understanding of how the molecule functions. The principal method for structure determination at near-atomic resolution is X-ray crystallography. Here, we demonstrate the current methods for obtaining three-dimensional crystals of any given macromolecule that are suitable for structure determination by X-ray crystallography.

Tags

Protein Crystallization X-ray Crystallography Biological Macromolecules Atomic Resolution Structures Protein Data Bank (rcsb-PDB) Macromolecular Structures Crystallographic Studies Purification Homogeneity High Resolution Crystals Supersaturation Concentration Aggregation Precipitation Nucleation Protein Crystals Vapor Diffusion Batch Crystallization
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