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Immunology and Infection

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JoVE Journal Immunology and Infection
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Article DOI: 10.3791/2540-v • 10:44 min • February 2nd, 2011
February 2nd, 2011 •
1, 1, 1, 1
1Division of Pediatrics, MD Anderson Cancer Center - University of Texas

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Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.

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Expansion Purification Functional Assessment Human Peripheral Blood NK Cells Natural Killer Cells Immune Surveillance Infectious Microorganisms Tumors Limited Availability In Vitro NK Cell Immunotherapy Method Efficiently Expand Vast Quantities Functional NK Cells Ex Vivo K562 Cells Expressing Membrane-bound IL21 Artificial Antigen-presenting Cell (aAPC) Cell Product Steady-state Leukapheresis Donor Depletion Of T Cells Positive Selection Of NK Cells IL-2 Overnight Activation Small Numbers Of NK Cells Delivered In Clinical Trials Inability To Propagate NK Cells In Vitro Generating Sufficient Cell Numbers
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