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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

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HeLa Based Cell Free Expression Systems Plasmodium Rhoptry Proteins Malaria Vaccine Candidates Prokaryotic Cell Expression Systems Eukaryotic Cell Expression Systems Glycosylation Immunogenicity Wheat Germ Expression System Rabbit Reticulocyte Lysate Expression System Escherichia Coli Lysate Expression System HeLa Based Cell Free Expression Systems In Vitro Human Cell Free Expression Systems Transcription And Translation Coupled Expression System Hydrophobic PF3D7 0114100 Maurer
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