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Immunology and Infection

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Isolation of Leukocytes from the Human Maternal-fetal Interface

Article DOI: 10.3791/52863-v • 08:19 min • May 21st, 2015
May 21st, 2015 •
1, 1, 1,2,3,4, 1,5, 1,5,6
1Perinatology Research Branch, NICHD/NIH/DHHS, 2Department of Obstetrics and Gynecology, University of Michigan, 3Department of Epidemiology and Biostatistics, Michigan State University, 4Department of Molecular Obstetrics and Genetics, Wayne State University, 5Department of Obstetrics and Gynecology, Wayne State University School of Medicine, 6Department of Immunology and Microbiology, Wayne State University School of Medicine

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Resumen
Automatic Translation

Described herein is a protocol to isolate and further study the infiltrating leukocytes of the decidua basalis and decidua parietalis - the human maternal-fetal interface. This protocol maintains the integrity of cell surface markers and yields enough viable cells for downstream applications as proven by flow cytometry analysis.

Tags

Isolation Leukocytes Human Maternal-fetal Interface Pregnancy Reproductive Tissues Decidua Basalis Decidua Parietalis Immunological Site Implantation Pregnancy Maintenance Timing Of Delivery Phenotypic Characterization Functional Characterization Pregnancy Disorders Protocols Reagents Enzymes Incubation Times Gentle Mechanical Dissociation Enzymatic Dissociation Viability Integrity Extracellular Markers Intracellular Markers Immunophenotyping Cell Culture Cell Sorting DNA Methylation Gene Expression
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