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Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Article DOI: 10.3791/54344-v • 10:51 min • August 30th, 2016
August 30th, 2016 •
1, 1, 1, 2, 2,3,4
1Department of Biomedical Engineering, The University of Texas at Austin, 2Wyss Institute for Biologically Inspired Engineering at Harvard University, 3Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, 4John A. Paulson School of Engineering and Applied Sciences, Harvard University

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We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

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Co-culture Living Microbiome Microengineered Human Intestinal Villi Gut-on-a-chip Microfluidic Device Villus Growth Peristalsis-like Motions Flow Co-culture With Gut Microbiome Gastroenterology Clinical Microbiology Pharmaceutical Sciences Disease Mechanism Efficacy And Toxicity Of New Drug Compounds Viable And Functional Post-microbiome Ecosystem Ulcerative Colitis Crohn's Disease Complex Interactions Between Gut Microbiome And Immune System Organ Systems Skin Genital Tract Oral Cavity
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