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Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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Resumen
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Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

Tags

Immunofluorescence Imaging DNA Damage Repair Foci Human Colon Cancer Cells Radiation-induced Foci Immunofluorescence Staining Neutron-mixed Beam Repair Pathway Proteins DNA Damaging-agents Ionizing Radiation Boron Neutron Capture Therapy BNCT Radiation-induced DNA Damage Response High-LET Beams Protons Proton Beam Therapy Carbon Ions Hadron Therapy Medium Removal PBS Wash Ethanol Fixation Cell Permeabilization Triton X-100 BSA Blockage Primary Antibody Staining
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