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JoVE Journal Biochemistry
Fluorescensbaserte målinger av fosfatidylserin/fosfatidylinositol 4-fosfatutveksling mellom membraner
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Fluorescensbaserte målinger av fosfatidylserin/fosfatidylinositol 4-fosfatutveksling mellom membraner

Article DOI: 10.3791/62177-v • 08:49 min • March 14th, 2021
March 14th, 2021 •
*1, *1, 1, 1
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
* These authors contributed equally

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Her beskriver vi protokoller ved hjelp av fluorescerende lipidsensorer og liposomer for å avgjøre om et proteinekstrakter og transporterer fosfatidylserin eller fosfatidylinositol 4-fosfat in vitro.

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Biokjemi Utgave 169 lipidoverføringsprotein OSBP-relatert protein fosfatidylserin fosfatidylinositol 4-fosfat fluorescens rekombinant protein liposom
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