Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

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Neuroscience
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JoVE Journal Neuroscience

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

Article DOI: 10.3791/62917-v • 07:46 min • January 22nd, 2022

January 22nd, 2022 •

This proctocol aims to provide a method for in vitro and in vivo mitochondrial Ca²⁺ imaging in astrocytes and neurons.

Imaging Mitochondrial Ca2+ Uptake Astrocytes Neurons Genetically Encoded Ca2+ Indicators (GECIs) Zhe Zhang Nannan Zhang Illker Ozden Shinghua Ding Cell Type Specific Pair In Vitro In Vivo Mitochondrial Targeting Method DNA Plasmids Astrocyte Specific Promoter GfaABC1D Neuron Specific Promoter CaMKII GCaMP5G GCaMP6s Lipofectamine Method Fluorescent Signals Epifluorescence Microscope Two-photon Microscope Serotype 5 Associated Adenovirus Serotype 9 Associated Adenovirus Stereotaxic Virus Injection Surgeries