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JoVE Journal Biochemistry
Zweifarbige Fluoreszenz-Kreuzkorrelationsspektroskopie zur Untersuchung der Protein-Protein-Interaktion und Proteindynamik in lebenden Zellen
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Journal (Biochemistry)
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
Journal (Biochemistry)
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
 
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Zweifarbige Fluoreszenz-Kreuzkorrelationsspektroskopie zur Untersuchung der Protein-Protein-Interaktion und Proteindynamik in lebenden Zellen

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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Wir präsentieren ein experimentelles Protokoll und einen Datenanalyse-Workflow zur Durchführung der zweifarbigen Fluoreszenz-Kreuzkorrelationsspektroskopie (FCCS) in Kombination mit Förster Resonance Energy Transfer (FRET), um die Membranrezeptordynamik in lebenden Zellen mit modernen Fluoreszenzmarkierungstechniken zu untersuchen.

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Biochemie Heft 178
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