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JoVE Journal Biochemistry
Tofarget fluorescens krysskorrelasjonsspektroskopi for å studere proteinproteininteraksjon og proteindynamikk i levende celler
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Tofarget fluorescens krysskorrelasjonsspektroskopi for å studere proteinproteininteraksjon og proteindynamikk i levende celler

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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Vi presenterer en eksperimentell protokoll- og dataanalysearbeidsflyt for å utføre live celle dual-color fluorescens krysskorrelasjonsspektroskopi (FCCS) kombinert med Förster Resonance Energy transfer (FRET) for å studere membranreseptordynamikk i levende celler ved hjelp av moderne fluorescensmerkingsteknikker.

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