Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Inner Mitochondrial Membrane Sensitivity to Na+…
JoVE Journal
Biochemistry

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Biochemistry
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Journal (Biochemistry)
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Cerebral Thrombus Clot Dissolution and Cell Staining for Cell Component Analyses (Video) | JoVE
Journal (Biochemistry)
Cerebral Thrombus Clot Dissolution and Cell Staining for Cell Component Analyses (Video) | JoVE
 

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Article DOI: 10.3791/63729-v • 05:27 min • July 20th, 2022
July 20th, 2022 •
1, 1,2
1Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, 2Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

Capítulos

Resumen
Automatic Translation

This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na+, to study the existence of partially segmented functional CoQ pools.

Tags

Inner Mitochondrial Membrane Na+ Sensitivity Functional CoQ Pools Sodium As A Second Messenger Ubiquinol Pools Electron Transfer Lipid Drops Sample Splitting Labeling Buffer Preparation Temperature Control Cytochrome C Malonate Potassium Chloride Sodium Chloride Rotenone NADH Spectrophotometer Measurement
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter