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Journal / Bioengineering / Lysinduceret dielektroforese til karakterisering…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Lysinduceret dielektroforese til karakterisering af den elektriske opførsel af humane mesenkymale stamceller
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Journal (Bioengineering)
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Lysinduceret dielektroforese til karakterisering af den elektriske opførsel af humane mesenkymale stamceller

Article DOI: 10.3791/64909-v • 10:08 min • June 16th, 2023
June 16th, 2023 •
1, 1, 2, 1, 1, 1,2,3,4, 1,2,4
1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering, University of California, Irvine, 3Department of Chemistry, School of Physical Sciences, University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center, University of California, Irvine

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Her præsenterer vi lysinduceret dielektroforese som en etiketfri tilgang til karakterisering af heterogene cellelinjer, specifikt humane mesenkymale stamceller (hMSC'er). Dette papir beskriver en protokol til brug og optimering af en mikrofluidisk enhed med et fotoledende lag til at karakterisere hMSC'ers elektriske opførsel uden at ændre deres oprindelige tilstand.

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Denne måned i JoVE udgave 196 Elektrokinetik mikrofluidik stamceller virtuelle elektroder cellekarakterisering
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