Generación y análisis transcriptómico unicelular de organoides de carcinoma hepatocelular tras el tratamiento con medicamentos

497 visualizaciones

08:18 min.

26 de mayo de 2026

497 visualizaciones

Dan He^*¹ , Zhong Liang^*¹^,²^,³ , Cheng-Yang Zhang^*¹^,²^,³ , Jie-Shen Xian^*⁴ , Yuan-Xiang Yang⁵ , Yuan-Feng Gong³ , Hui Tang³ , Guang-Ding Zhou¹^,²^,³ , Chen-Yu Jiang¹^,²^,³ , Le Xing¹^,²^,³ , Dong-Mei Li¹^,²^,³ , Ying-Chen Hou¹^,²^,³ , Chen Yue¹^,²^,³ , Wei Cheng¹^,² , Yun-Qiang Tang³ , Ming Liu¹^,²^,³ , Xiao-Feng Zhang¹^,²^,³^,⁶

¹School of Basic Medical Science, Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Guangzhou Medical University, ³Affiliated Cancer Hospital and Institute, Guangzhou Medical University, ⁴The First Clinical School of Guangzhou Medical University, Guangzhou Medical University, ⁵Department of Obstetrics, Guangzhou Eighth People's Hospital, Guangzhou Medical University, ⁶Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University

^* These authors contributed equally

Este protocolo describe un método simplificado para generar organoides de carcinoma hepatocelular, aplicar tratamientos farmacológicos y realizar secuenciación de ARN unicelular antes y después del tratamiento para caracterizar los cambios transcripcionales asociados al tratamiento.