Sun Yat-sen University 22 articles published in JoVE Medicine Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy Yiming Song*1, Peiran Li*2, Ping Lyu1, Yanshen Yu1, Xinyu Chen1, Chen Cui3, Ruiye Bi2, Yi Fan1 1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, 2State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthognathic and TMJ Surgery, West China Hospital of Stomatology, 3Guangdong Province Key Laboratory of Stomatology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University An animal model of acquired hypoparathyroidism (HypoPT) is crucial to understanding how HypoPT affects mineral ion homeostasis and to verify the effectiveness of novel treatments. Here, a technique is presented to generate an acquired hypoparathyroidism (AHypoPT) rat model by parathyroidectomy (PTX) using carbon nanoparticles. Neuroscience A Procedure for Mouse Dorsal Root Ganglion Cryosectioning Liangliang He*1,2, Wenxing Zhao*1, Lingyi Zhang2,3, Maalveka Ilango2,4, Na Zhao2, Liqiang Yang1, Zhonghui Guan2 1Department of Pain Management, Xuanwu Hospital, Capital Medical University, 2Department of Anesthesia and Perioperative Care, University of California San Francisco, 3Department of Anesthesiology, Sun Yat-Sen University, 4University of Washington Presented here is the development for consistently acquiring high-quality dorsal root ganglion cryostat sections. Medicine Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis Dawei Zhang*1, Zuxiao Chen*2, Lei Zhang1, Xiangjun Qian1, Xiaoming Huang1, Zheyu Zheng1, Weidong Pan1 1Department of Pancreatic Hepatobiliary Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University This protocol describes the laparoscopic resection of colorectal cancer liver metastases combined with ultrasound-guided microwave ablation. This technique can safely, effectively, and accurately treat refractory liver metastases <3 cm, reduce postoperative complications, and accelerate the postoperative rehabilitation of patients. Medicine Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair Ning Ma*1, Dai Tang*2, Fu-xing Tang1, En-min Huang1, Tao Ma1, Wei-sheng Yang1, Chuang-xiong Liu1, Hao-nan Huang1, Shuang Chen1, Tai-cheng Zhou1 1Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, 2 Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain. Medicine Indocyanine Green-Guided Intraoperative Imaging to Facilitate Video-Assisted Retroperitoneal Debridement for Treating Acute Necrotizing Pancreatitis Li Huang1, Wei Chen1, Jiancong Chen1, Dong Chen1, Kunsong Zhang1, Jianpeng Cai1, Hong Peng1, Chensong Huang1, Guangyan Zeng1, Mingjian Ma1, Jiahua Liang1, Borui Xu1, Xiaoyu Yin1, Jiaming Lai1, Lijian Liang1 1Department of Pancreatobiliary Surgery, The First Affiliated Hospital, Sun Yat-sen University This protocol presents Indocyanine Green-guided video-assisted retroperitoneal debridement (ICG-guided VARD) for treating severe acute necrotizing pancreatitis. Cancer Research Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice Qibin Fu1, Yan Zhang1, Tuchen Huang1, Yang Liu1 1Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice. Biology Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells Hongzi Chen1, Pan Huang1, Yan Zhang1 1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells. Medicine Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors Dongjie Yang*1,2, Xun Hou*1, Wentong Lan*3, Minxuan Shen1, Shaohua Yang4, Lele Zhang1, Qinbo Cai1, Yan Qian1, Yuan Lin5, Xia Feng6, Yulong He1,2,4 1Center for Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, 2Guangdong Provincial Key Laboratory of Digestive Cancer Research, 3Department of Endoscopy, The First Affiliated Hospital, Sun Yat-sen University, 4Digestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, 5Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 6Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their drawbacks. Cancer Research Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice Lisha Wang1, Zhiming Wang1, Junyi Guo2, Huayu Lin1, Shuqiong Wen2, Qiao Liu1, Yiding Li3, Qing Wu1, Leiqiong Gao1, Xiangyu Chen1, Luoyingzi Xie1, Qin Tian1, Jianfang Tang1, Zhirong Li1, Li Hu1, Juan Wang4, Lifan Xu1, Qizhao Huang5, Lilin Ye1 1Institute of Immunology, Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital, Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, 5Cancer Center, The General Hospital of Western Theater Command Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses. Medicine Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells Yuanyuan Guan*1, Bingbing Xie*1, Xiufeng Zhong1 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China, 510060 Here we describe an optimized retinal organoid induction system, which is suitable for various human pluripotent stem cell lines to generate retinal tissues with high reproducibility and efficiency. Biology Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model Yuan Ma1, Tao Li1 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University This protocol describes a detailed method for the preparation and immunofluorescence staining of mice retinal flat mounts and analysis. The use of fluorescein fundus angiography (FFA) for mice pups and image processing are described in detail as well. Biology Detecting Migration and Infiltration of Neutrophils in Mice Qingyi Lu*1, Kai Yuan*1, Xiaohong Li1, Haixu Jiang1, Guiyang Huo1, Wenrui Jia1, Guangrui Huang1, Anlong Xu1,2 1School of Life Sciences, Beijing University of Chinese Medicine, 2State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University Here, we present three methods to assess neutrophil migration and infiltration both in vivo and in vitro. These methods can be used to discover promising therapeutics targeting neutrophil migration. Biology Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry Dianlei Guo1, Chunqiao Liu1 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University This protocol describes a method for the isolation of the mouse eyeball with eyelid, ocular surface, anterior and posterior segments in relatively intact position. Cancer Research Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma Yu Zhang1, Yupeng Yao1, Yan Zhang1 1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS. Chemistry Efficient Construction of Drug-like Bispirocyclic Scaffolds Via Organocatalytic Cycloadditions of α-Imino γ-Lactones and Alkylidene Pyrazolones Yani Zhou1, Nannan Chen2, Yaping Cheng2, Xiaoqing Cai2 1School of Basic Medical Sciences, Lanzhou University, 2School of Pharmaceutical Sciences, Sun Yat-sen University Enantiomerically enriched bispiro[γ-butyrolactone-pyrrolidin-4,4'-pyrazolone] skeletons are asymmetrically synthesized through a simple organocatalytic 1,3-dipolar cycloaddition reaction. Engineering Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons Zhaolong Cao1,2, Min Lin2, Daniel Ong2 1State Key Laboratory of Optoelectronic Materials and Technologies and Guangdong Province Key Laboratory of Display Material and Technology, School of Physics and Engineering, Sun Yat-sen University, 2Department of Physics, The Chinese University of Hong Kong This protocol describes the instrumentation for determining the excitation and coupling rates between light emitters and Bloch-like surface plasmon polaritons arising from periodic arrays. Cancer Research A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity Yanlan Wang1,2, Jiayu Liu1,2, Haitao Pan1,2, Jieyu Xing1,2, Xiaoqiong Wu1,2, Qing Li1,2, Zhong Wang1,2 1School of Pharmaceutical Sciences, Sun Yat-Sen University, 2Center for Cellular & Structural Biology, Sun Yat-Sen University Here, we present a protocol to produce a bispecific antibody GPC3-S-Fab in Escherichia coli. The purified GPC3-S-Fab has potent cytotoxicity against GPC3 positive liver cancer cells. Immunology and Infection A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals Jingshu Chen*2, Lei Zhong*1, Jing Wu1, Sui Ke2, Benjamin Morpurgo3, Andrei Golovko3, Nengtai Ouyang4, Yuxiang Sun2, Shaodong Guo2, Yanan Tian1,2 1Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agriculture University, 2Texas A&M University, 3Texas A&M Institute for Genomic Medicine, 4Sun Yat-Sen Memorial Hospital Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic chemicals. Cancer Research Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro Yupeng Yao1, Weifan Lin1, Yan Zhang1 1Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions. Behavior Bouncing Ball with a Uniformly Varying Velocity in a Metronome Synchronization Task Yingyu Huang*1, Li Gu*1, Junkai Yang*1, Xiang Wu1 1Department of Psychology, Sun Yat-Sen University The purpose of this protocol is to introduce the application of a bouncing ball with a uniformly varying velocity in a metronome synchronization task. Environment Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS Zhiyong Lin1,3, Xiaoming Sun1,2,3,4, Jörn Peckmann5, Yang Lu2,3, Harald Strauss6, Li Xu2,3, Hongfeng Lu7, Barbara M.A. Teichert6 1School of Earth Sciences and Engineering, Sun Yat-sen University, 2School of Marine Sciences, Sun Yat-sen University, 3Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, 4South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, 5Institut für Geologie, Universität Hamburg, 6Institut für Geologie und Paläontologie, Westfälische Wilhelms-Universität Münster, 7Guangzhou Marine Geological Survey Analyses of the sulfur isotopic composition (δ34S) of pyrite from methane-bearing sediments have typically focused on bulk samples. Here, we applied secondary ion mass spectroscopy to analyze the δ34S values of various pyrite generations to understand the diagenetic history of pyritization. Bioengineering Porous Silicon Microparticles for Delivery of siRNA Therapeutics Jianliang Shen*1,2, Xiaoyan Wu*1,3, Yeonju Lee1, Joy Wolfram1,4, Zhizhou Yang1, Zong-Wan Mao2, Mauro Ferrari1,5, Haifa Shen1,6 1Department of Nanomedicine, Houston Methodist Research Institute, 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, 3Pediatrics Department of Union Hospital, Huazhong University of Science and Technology, 4CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, 5Department of Medicine, Weill Cornell Medical College, 6Department of Cell and Developmental Biology, Weill Cornell Medical College Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon microparticles.
