Henan University of Science and Technology 1 article published in JoVE Developmental Biology Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species Qiongxia Lyu1,2, Huifei Sophia Zheng2, Karly Laprocina2, Chen-Che Jeff Huang2 1College of Animal Science & Technology, Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University Several different methods have been established for multiplex immunostaining using primary antibodies from the same host species. Here, we describe the use of microwave-mediated antibody stripping and of fluorophore-tyramide to block antibody cross-reactivity during multiplex immunostaining on formalin-fixed paraffin-embedded mouse adrenal sections.