Shanghai Polytechnic University 1 article published in JoVE Environment Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae Bin Zhang1,2, Xinyue Yang2, Jing Zhao3, Ting Xu2,4, Daqiang Yin2,4 1State Key Laboratory of Marine Geology, Tongji University, 2Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 3Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of 10 Shanghai Polytechnic University, 4Shanghai Institute of Pollution Control and Ecological Security A detailed experimental protocol is presented in this paper for the evaluation of neurobehavioral toxicity of environmental pollutants using a zebrafish larvae model, including the exposure process and tests for neurobehavioral indicators.