Hong Kong Polytechnic University View Institution's Website 7 articles published in JoVE Engineering An Available Technique for Preparation of New Cast MnCuNiFeZnAl Alloy with Superior Damping Capacity and High Service Temperature Dong Li1,3, Wenbo Liu1,2, Ning Li1, Zhenyu Zhong1, Jiazhen Yan1, Sanqiang Shi2 1School of Manufacturing Science and Engineering, Sichuan University, 2Department of Mechanical Engineering, Hong Kong Polytechnic University, 3Department of Mechanical and Electrical Engineering, Chengdu Aeronautic Polytechnic Here we present a protocol to obtain a novel Mn-Cu-based alloy with excellent comprehensive performances by a high-quality smelting technology and reasonable heat treatment methods. Engineering Experimental Investigation of the Flow Structure over a Delta Wing Via Flow Visualization Methods Lu Shen1, Zong-nan Chen1, Chihyung Wen1 1Department of Mechanical Engineering, Hong Kong Polytechnic University Here, we present a protocol to observe unsteady vortical flows over a delta wing using a modified smoke flow visualization technique and investigate the mechanism responsible for the oscillations of the leading-edge vortex breakdown locations. Neuroscience Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture Lin Jiajia*1, Mak Shinghung*2, Zheng Jiacheng1, Wang Jialing1, Xu Dilin1, Hu Shengquan2, Zhang Zaijun3, Wang Qinwen1, Han Yifan2,4, Cui Wei1,2 1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research. Chemistry TiO2-coated Hollow Glass Microspheres with Superhydrophobic and High IR-reflective Properties Synthesized by a Soft-chemistry Method Yinting Wong1, Dan Zhong2, Aotian Song4, Yan Hu3 1Department of Biomedical Science, City University of Hong Kong, 2Technological and Higher Education Institute of Hong Kong, 3Hong Kong Polytechnic University, 4Pavotech Co. Ltd. This manuscript proposes a soft-chemistry method to synthesize superhydrophobic, TiO2-coated hollow glass microspheres (HGM) with high IR-reflective properties. Medicine Minimally Invasive Transverse Aortic Constriction in Mice Aung Moe Zaw1, Connor M. Williams2, Helen K. W. Law3, Billy Kwok Chong Chow1 1School of Biological Sciences, The University of Hong Kong, 2College of Arts and Science, New York University, 3Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University Minimally invasive transverse aortic constriction (MTAC) conserves the essentials of regular transverse aortic constriction (TAC) while eliminating the use of a ventilator with tracheal intubation. It proves to be a highly desirable method for high-throughput studies on left ventricular overload, particularly in translational studies. Genetics In Vitro Transcription Assays and Their Application in Drug Discovery Xiao Yang1, Cong Ma1,2 1School of Environmental and Life Sciences, University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription. Biology Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure Kenneth K. Kwong1,2, Lenuta Kloetzer1,2,3,4, Kelvin K. Wong5,6, Jia-Qian Ren1,2, Braden Kuo1,2,3,4, Yan Jiang7, Y. Iris Chen1,2, Suk-Tak Chan1,2,8, Geoffrey S. Young9, Stephen T.C. Wong5,6 1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, 4Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 5Center for biotechnology and Informatics, The Methodist Hospital Research Institute, 6Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, 7Bejing University of Chinese Medicine, 8Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 9Department of Radiology, Brigham and Women's Hospital, Harvard Medical School Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.