Kobe University Graduate School of Medicine 1 article published in JoVE Biology Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells Katsuyuki Nishi1,2, Akiomi Nagasaka1,2, Taro Sakamaki1,2, Kay Sadaoka1,2, Masanori Miyanishi1,2 1Hematopoietic Stem Cell Biology and Medical Innovation (HSCBMI), Department of Pediatrics, Kobe University Graduate School of Medicine, 2RIKEN Center for Biosystems Dynamics Research We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.