Universite Paris-Cite, Institut Pasteur, Equipe Labellisee Ligue Contre le Cancer 1 article published in JoVE Biology Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain Florent Peglion1, Franck Coumailleau1, Sandrine Etienne-Manneville1 1Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.
