University of Hartford 2 articles published in JoVE Genetics The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression Adam C. Silver1 1Department of Biology, University of Hartford This protocol describes a technique using mouse splenocytes to discover pathogen-associated molecular patterns that alter molecular clock gene expression. Bioengineering Force System with Vertical V-Bends: A 3D In Vitro Assessment of Elastic and Rigid Rectangular Archwires Madhur Upadhyay1, Raja Shah2, Sachin Agarwal3, Meenakshi Vishwanath4, Po-Jung Chen5, Takafumi Asaki6, Donald Peterson7 1Division of Orthodontics, University of Connecticut Health, 2Private Practice, 3Department of Orthodontics, University of Melbourne, 4Department of Orthodontics, University of Nebraska Medical Center, 5Department of Craniofacial Sciences, University of Connecticut Health, 6Biomedical Engineering, University of Hartford, 7Department of Mechanical Engineering, College of Engineering and Engineering Technology, Northern Illinois University The method presented here is designed to construct and validate an in vitro 3D model capable of measuring the force system generated by different archwires with V-bends placed between two brackets. Additional objectives are to compare this force system with different types of archwires and to previous models.