Kansas City Veterans Affairs Medical Center 1 article published in JoVE Neuroscience Stereological Estimation of Cholinergic Fiber Length in the Nucleus Basalis of Meynert of the Mouse Brain Prabhakar Singh1, David W. Peng1, William Z. Suo1,2,3,4 1Laboratory for Alzheimer's Disease and Aging Research, Kansas City Veterans Affairs Medical Center, 2Department of Neurology, University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 4 Neuronal fiber length within a three-dimensional structure of a brain region is a reliable parameter to quantify specific neuronal structural integrity or degeneration. This article details a stereological quantification method to measure cholinergic fiber length within the nucleus basalis of Meynert in mice as an example.