Summary
Here's a look at what's coming up in the June 2017 issue of JoVE: The World's Premier Video Journal
Protocol
Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
Sangyun Jeong
Department of Molecular Biology, Chonbuk National University
This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
Wenting Luo*1,2,3,4, Guichang Pan*5, Huimin Huang1,2,3,4, Peiyan Zheng1,2,3,4, Nili Wei1,2,3,4, Yifei Zhang5, Guangqiao Zeng1,2,3,4, Baoqing Sun1,2,3,4
1State Key Laboratory of Respiratory Disease, 2National Clinical Research Center of Respiratory Disease, 3Guangzhou Institute of Respiratory Diseases, 4Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, 5Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Marlene K. Wolfe, Daniele S. Lantagne
Department of Civil and Environmental Engineering, Tufts University
Handwashing is widely recommended to prevent infectious disease transmission. However, there is little evidence on which handwashing methods are most efficacious at removing infectious disease pathogens. We developed a method to assess the efficacy of handwashing methods at removing microorganisms.
Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
Xun Zhang, Sheng Chen, Feng Xu
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University
This protocol aims to present a general method to visualize lignin, cellulose, and hemicellulose in plant cell walls using Raman imaging and multivariate analysis.
Disclosures
No conflicts of interest declared.