Protocol
A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting
Linda S. Franck1,2, Monica R. McLemore1,2, Norlissa Cooper1, Baylee De Castro3, Anastasia Y. Gordon4, Schyneida Williams5, Shanell Williams2, Larry Rand2,3
1School of Nursing, University of California, San Francisco, 2UCSF California Preterm Birth Initiative, University of California, San Francisco, 3School of Medicine, University of California, San Francisco, 4San Francisco Black Infant Health Program, 5Homeless Prenatal Program, San Francisco, CA
This manuscript describes the Research Prioritization by Affected Communities (RPAC) protocol and findings from its use with women at risk for preterm birth. Using the protocol, women identified and prioritized their unanswered questions about pregnancy, birth and neonatal care aimed at influencing research priority setting by funders and researchers.
Frame-by-Frame Video Analysis of Idiosyncratic Reach-to-Grasp Movements in Humans
Jenni M. Karl1, Jessica R. Kuntz2, Layne A. Lenhart2, Ian Q. Whishaw2
1Department of Psychology, Thompson Rivers University, 2Department of Neuroscience, University of Lethbridge
This protocol describes how to use frame-by-frame video analysis to quantify idiosyncratic reach-to-grasp movements in humans. A comparative analysis of reaching in sighted versus unsighted healthy adults is used to demonstrate the technique, but the method can also be applied to the study of developmental and clinical populations.
Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
Ming J. Cheng1, Priya Prabakaran1, Rajiv Kumar2,3, Srinivas Sridhar1,2,3, Eno E. Ebong1,4,5
1Department of Chemical Engineering, Northeastern University, 2Nanomedicine Science and Technology Center, Northeastern University, 3Department of Physics, Northeastern University, 4Departments of Bioengineering, Northeastern University, 5Department of Neuroscience, Albert Einstein College of Medicine
We describe a method of synthesizing biocompatible 10-nm gold nanoparticles, functionalized by coating poly-ethylene glycol onto the surface. These particles can be used in vitro and in vivo for delivering therapeutics to nanoscale cellular and extracellular spaces that are difficult to access with conventional nanoparticle sizes.
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Lin Jin*1,2, Aaron E. Engelhart*1,3, Katarzyna P. Adamala1,3, Jack W. Szostak1
1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 2Department of Biomedical Engineering, Boston University, 3Department of Genetics, Cell Biology, and Development, University of Minnesota
Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.
Disclosures
No conflicts of interest declared.
