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Immunology and Infection

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Isolation of Brain-infiltrating Leukocytes

Article DOI: 10.3791/2747-v • 06:44 min • June 13th, 2011
June 13th, 2011 •
*1, *1
1Department of Neurology, Mayo Clinic College of Medicine
* These authors contributed equally

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résumé
Automatic Translation

A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.

Tags

Brain-infiltrating Leukocytes BILs Mice Theiler's Murine Encephalomyelitis Virus TMEV Intracranial Injection Technique Leukocyte Purification Brain Homogenization Percoll Gradient Cell Suspension Ficoll-Paque Plus Gradient Immunophenotyping Flow Cytometry Innate Immune Cells Gr1+ CD11b+ F4/80+ Cells Immune Response Acute Infection
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