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Immunology and Infection

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JoVE Journal Immunology and Infection
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Article DOI: 10.3791/3293-v • 08:53 min • September 28th, 2011
September 28th, 2011 •
1, 1, 2, 3
1Department of Pediatrics, University of Texas Southwestern Medical Center, 2Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 3Department of Pediatrics and Microbiology, University of Texas Southwestern Medical Center

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A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.

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RNA Isolation Pseudomonas Aeruginosa Gastrointestinal Tract Colonization Immune Systems Leukemia Burn Wounds Organ Transplants Bloodstream Infections Transcription Profiling MRNA Gene Expression Immune Status Isolation High Quality MRNA Enzymes Debris Food Residues Particulate Matter GI Colonization Neutropenia-induced Dissemination Mice Euthanization
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