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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Article DOI: 10.3791/51604-v • 06:36 min • July 17th, 2014
July 17th, 2014 •
1, 1, 1, 1, 1
1Department of Biological Sciences, University of Notre Dame

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The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.

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Flat Mount Preparation Observation Analysis Zebrafish Embryo Stained Whole Mount In Situ Hybridization Genetic Control Developmental Processes Congenital Abnormalities Developmental Stages Fertilization Cleavage Gastrulation Segmentation Organogenesis Kidney Heart Central Nervous System Visualization Analysis Tissues Yolk Mass Experimental Phenotypes Fixed Embryonic Specimens Tailbud Stage Somite Stage Hours Post Fertilization (hpf) Glass Slide Flat Mount Procedure Dissection Technique Reagents Gene Expression Detection
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