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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Article DOI: 10.3791/52350-v • 11:27 min • February 7th, 2015
February 7th, 2015 •
1, 1, 1, 1, 2, 1
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine, 2Seahorse Biosciences

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résumé
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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

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Preparation Respirometric Assessment Mitochondria Skeletal Muscle Tissue Percutaneous Needle Biopsy Electron Transport Chain Function Human Vastus Lateralis Plate-based Respirometric Profiling Extracellular Flux (XF) Analyzer Comparison Respirometric Profiles Mitochondria Amount Respiration Measurement Consistent Results Standard Errors Western Blot Analysis Mitochondrial Marker COX IV Non-mitochondrial Tissue Marker GAPDH Non-mitochondrial Contamination Clinical Research Projects
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