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Immunology and Infection

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JoVE Journal Immunology and Infection
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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Article DOI: 10.3791/54296-v • 08:31 min • October 18th, 2016
October 18th, 2016 •
1, 1, 1, 1, 2, 1, 1
1Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2Millipore Sigma

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This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

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Human Monocytes Dendritic Cells Imaging Flow Cytometry Monocyte Isolation Protocols CT11c Expression CT14 Expression Reliable Methods Purification Viable And Functional Cells Single Cell Imaging Flow Cytometry Rare Cells Isolation Blood Sample Dilution PBS Density Gradient Solution
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