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Developmental Biology

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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Article DOI: 10.3791/55914-v • 10:03 min • August 1st, 2017
August 1st, 2017 •
*1, *1, 1, 1, 1
1Haematopoietic Stem Cell Laboratory, The Francis Crick Institute
* These authors contributed equally

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A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.

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Bioengineering Humanized Bone Marrow Microenvironments Mouse Visualization Live Imaging Human Cell Carrier Scaffolds Single Cell Resolution Bone Marrow Niche Components Tumor Metastasis Drug Screening Studies Gelatin Sponge Sterilized Reconstitution Ethanol PBS Ultra Low Attachment 24-well Plate Stromal Cells Culture Medium
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