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A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Article DOI: 10.3791/57800-v • 11:28 min • August 25th, 2018
August 25th, 2018 •
*1,2, *1,3, 1, 4, 1,2, 1, 1,5
1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE, CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group
* These authors contributed equally

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résumé
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A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.

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Hydroponic System Plant Responses Small Molecules Sterile Conditions In Vitro Large Scale Experiments Chemicals In Solution Efficient Absorption Molecular Studies Biochemical Studies Physiological Studies Arabidopsis Thaliana Seeds Ethanol Bleach Solution Polysorbate 20 MS Medium MES Monohydrate Agar Potassium Hydroxide Laminar Flow Hood Hot Plate Growth Chamber Disposable Plastic Boxes Glass Petri Dishes Adhesive Tape Pipet Tips Scissors Multichannel Pipet Scalpel Blade Tweezers
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