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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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résumé
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A detailed protocol is described for the separation, identification, and characterization of proteoforms in protein samples using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS). The protocol can be used for the high-resolution characterization of proteoforms in simple protein samples and the large-scale identification of proteoforms in complex proteome samples.

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Large-scale Top-down Proteomics Capillary Zone Electrophoresis Tandem Mass Spectrometry Protein Isoforms Protein Post-translational Modifications Efficient Separation Sensitive Detection Capillary Treatment Polyacrylamide Coating Capillary Threading HF Treatment
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