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Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Automatic Translation

This work presents a protocol to yield homogeneous cell cultures of primary oculomotor, trochlear, and spinal motor neurons. These cultures can be used for comparative analyses of the morphological, cellular, molecular, and electrophysiological characteristics of ocular and spinal motor neurons.

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Oculomotor Trochlear Spinal Motor Neurons Isolation Culture Prenatal Islmn:GFP Transgenic Mice Primary Motor Neurons Embryonic Mice Motor Neuron Disease In Vitro Culture Amyotrophic Lateral Sclerosis Disease Mechanism Therapy Motor Neuron Development Selective Vulnerability Characterization Morphology Molecular Biology Electrophysiology Dissection Technique
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