University of Nairobi 1 article published in JoVE Biology Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology Criselda Bautista1,2, Gurdeep Jaswant1,3,4,5, Hollie French1,6, Kathryn Campbell1, Rowan Durrant1, Robert Gifford1,6, Grace S. N. Kia7,8, Brian Ogoti3,9, Katie Hampson1, Kirstyn Brunker1,6 1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi Here, we present a rapid and cost-effective workflow for characterizing rabies virus (RABV) genomes using nanopore technology. The workflow is intended to support genomics-informed surveillance at a local level, providing information on circulating RABV lineages and their placement within regional phylogenies to guide rabies control measures.
