Suez Canal University

1 article published in JoVE

• Genetics

  
  Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing Ahmed Elaswad*^1,2, Karim Khalil*^1,3, David Cline¹, Patrick Page-McCaw⁴, Wenbiao Chen⁴, Maximilian Michel⁵, Roger Cone⁵, Rex Dunham^{1 1}School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, ²Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, ³Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, ⁴Department of Molecular Physiology and Biophysics, Vanderbilt University, ⁵Life Science Institute, University of Michigan A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.