2 articles published in JoVE
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing Peibo Wang*1, Yongcan Zhou*1,3, Bei Wang2, Ahmed Elaswad4, Shifeng Wang1,3, Weiliang Guo1,3, Dongdong Zhang1,3 1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing Ahmed Elaswad*1,2, Karim Khalil*1,3, David Cline1, Patrick Page-McCaw4, Wenbiao Chen4, Maximilian Michel5, Roger Cone5, Rex Dunham1 1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.