University of the Free State 3 articles published in JoVE Medicine Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg Christian Klopsch1, Alexander Kaminski1, Friedrich Prall2, Pascal Dohmen1,3 1Department of Cardiac Surgery, Heart Center Rostock, Rostock University Medical Center, University of Rostock, 2Institute of Pathology, Rostock University Medical Center, University of Rostock, 3Department of Cardiothoracic Surgery, Faculty of Health Science, University of the Free State Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting. Immunology and Infection Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions Uju L. Madu1, Olihile M. Sebolai1 1Department of Microbial, Biochemical and Food Biotechnology, University of the Free State This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information. Environment Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri Charlotte Philippe1,2, Arnout F. Gregoir1, Eli S. J. Thoré1, Gudrun De Boeck2, Luc Brendonck1,3, Tom Pinceel1,4 1Animal Ecology, Global Change and Sustainable Development, University of Leuven, 2Systemic Physiological and Ecotoxicological Research, University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management, North-West University, 4Centre for Environmental Management, University of the Free State In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
