Shandong Academy of Agricultural Sciences 5 articles published in JoVE Behavior Dissection and Grading of Ovarian Development in Wild-Type Female Insects Lara Sindhu1, Sen Guo1, Yingying Song1, Lili Li1, Hongyign Cui1, Wenxiu Guo1, Suhong Lv1, Yi Yu1, Xingyuan Men1 1Institute of Plant Protection, Shandong Academy of Agricultural Sciences The protocol demonstrates a simple and easy dissection method, suitable for wild-type migratory female insects captured with searchlight traps. This technique can significantly clarify the same species by comparing both reproductive tissues, namely the mating sac and ovarian development of wild-type female insects. Biochemistry Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees Yingying Song1, Ruiping Li1,2, Lili Li1, Fang Ouyang3, Xingyuan Men1 1Institute of Plant Protection, Shandong Academy of Agricultural Sciences, 2College of Plant Protection, Shandong Agricultural University, 3State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences The present protocol explains a method to feed pesticide-contaminated provisions to the larvae of the solitary bees, Osmia excavata. The procedure examines the ecotoxicity of the pesticide to the larvae of the solitary bees. Neuroscience Single Sensillum Recordings for Locust Palp Sensilla Basiconica Hongwei Li1, Yinwei You2, Long Zhang1 1Department of Entomology, China Agricultural University, 2Bio-tech Research Center, Shandong Academy of Agricultural Sciences This paper describes a detailed and highly effective protocol for single sensillum recordings from the sensilla basiconica on the palps of insect mouthparts. Neuroscience Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization Xiao Xu1, Yinwei You1,2, Long Zhang1 1Department of Entomology, China Agricultural University, 2Bio-tech Research Center, Shandong Academy of Agricultural Sciences This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes. Biology Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus Najie Zhu*1, Liqun Bai*1, Stefan Schütz2, Baojun Liu3, Zhenyu Liu3, Xingyao Zhang4, Hongshi Yu1, Jiafu Hu1,5 1Department of Forest Protection, Zhejiang Agricultural & Forestry University, 2Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, 3College of Plant Protection, Shandong Agricultural University, 4Institute of Forest Protection, Chinese Academy of Forestry, 5Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.
