Huazhong Agricultural University 3 articles published in JoVE Biology Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses Muhammad Husnain Ahmad1, Xin Zheng1, Yu Hu1, Huimin Liu1, Yi Sun1, Hao Wen1, Lijun Chai1 1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University This protocol provides a rapid method for determining pollen compatibility and incompatibility in citrus cultivars. Genetics QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii Bang Shen1, Robin H. Powell2, Michael S. Behnke2 1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene. Biology Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana Juan Du1,2, Hendrik Rietman1, Vivianne G. A. A. Vleeshouwers1 1Wageningen UR Plant Breeding, Wageningen University, 2Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, National Centre for Vegetable Improvement (Central China), Potato Engineering and Technology Research Centre of Hubei Province, Huazhong Agricultural University Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.
