China Three Gorges University 1 article published in JoVE Immunology and Infection Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin Dechun Zhang*1, Xianzhao Kan*2, Sarah Elizabeth Huss3, Lan Jiang2, Li-Qing Chen3, Yibing Hu4 1Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, 2The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, 3Department of Plant Biology, University of Illinois at Urbana-Champaign, 4College of Resources & Environmental Sciences, Nanjing Agricultural University A method of constructing a phylogenetic tree based on sequence homology of SWEETs from eukaryotes and SemiSWEETs from prokaryotes is described. Phylogenetic analysis is a useful tool for explaining the evolutionary relatedness between homologous proteins or genes from different organism groups.