Yantai Zestern Biotechnique Co. LTD 1 article published in JoVE Biochemistry High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB) Xiaoying Qi*1, Yunyun Zhang*2, Yuan Zhang1, Tianhui Ni3, Wenfeng Zhang2, Chunhua Yang1, Jia Mi1,4, Jiandi Zhang2,3, Geng Tian1 1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.
