The First Affiliated Hospital of Soochow University 7 articles published in JoVE Medicine A Modified Technique for Arteriovenous Fistula Construction in Rabbits Liu Zhen*1,2,3, Hou Guocun*3, Wang Xiaohe3, Hu Jingfang3, Li Jie3, Wei Minggang1,2 1Department of Traditional Chinese Medicine, The First Affiliated Hospital of Soochow University, 2Department of Nephrology, The First Affiliated Hospital of Soochow University, 3Department of Nephrology, Suzhou Science & Technology Town Hospital The present protocol proposes the creation of an arteriovenous fistula in rabbits using a modified no-touch technique. The technique involves the side-to-side anastomosis of the common carotid artery and external jugular vein without the dissection of the perivenous tissues or cutting off the artery. Biology A Mouse Model of Ankle-Subtalar Complex Joint Instability Shuo Wang*1,2, Peixin Liu*1,2, Chunzhuo Hua1,2, Hongtao Zhang1,2, Jia Yu1,2 1Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 2Orthopaedic Institute, Soochow University The ankle-subtalar complex joint (ASCJ) is the core of the foot and plays a key role in balance control in daily activities. Sports injuries often lead to instability in this joint. Here, we describe a mouse model of ligament transection-induced instability of the ASCJ. Medicine Establishment of a Mouse Severe Acute Pancreatitis Model using Retrograde Injection of Sodium Taurocholate into the Biliopancreatic Duct Xiaochong Zhou1, Haifeng Chen1, Xing Wei1, Yang He2,4,5, Chunfang Xu1, Zhen Weng2,3,4,6 1Department of Gastroenterology, the First Affiliated Hospital of Soochow University, 2MOE Engineering Center of Hematological Disease, Soochow University, 3Cyrus Tang Hematology Center, Soochow University, 4National Clinical Research Center for Hematologic Diseases, the First Affiliated Hospital of Soochow University, 5MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 6Collaborative Innovation Center of Hematology, Soochow University A mouse model of severe acute pancreatitis is described herein. The procedure presented here is very rapid, simple, and accessible, thereby potentially allowing the study of the molecular mechanisms and different therapeutic interventions in acute pancreatitis in a convenient way. Neuroscience Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation Ming Luo1, Xiangyue Tang1, Juehua Zhu2, Zhihua Qiu1, Yongjun Jiang1 1The Second Affiliated Hospital of Guangzhou Medical University, 2Department of Neurology, The First Affiliated Hospital of Soochow University Presented here is a protocol for establishing acute pontine infarction in a rat model via electrical stimulation with a single pulse. Biology Quantification of Proliferative and Dead Cells in Enteroids Hua-Shan Li*1, Shao-Fang Xu*1, Jian-Ying Sheng*1, Zhi-Hui Jiang1, Jing Wang1, Ning Ding1, Tao Wang1, Matthew A. Odenwald2, Jerrold R. Turner2,3, Wei-Qi He1, Hong Xu1, Juan-Min Zha1 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3 The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival. Biology In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability Ban-Ruo Li*1,2, Jia Wu*1,2, Hua-Shan Li1,2, Zhi-Hui Jiang1,2, Xiu-Min Zhou1,2, Cai-Hua Xu1,2, Ning Ding1,2, Juan-Min Zha1,2, Wei-Qi He1,2 1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo. Medicine Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots Lu Xu1, Qiwen Shen1, Zhongqi Mao2, L. James Lee3, Ouliana Ziouzenkova1 1Department of Human Sciences, The Ohio State University, 2Department of Minimally Invasive Surgery, The First Affiliated Hospital of Soochow University, 3NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University Here, we present a protocol for encapsulation of catabolic cells, which consume lipids for heat production in intra-abdominal adipose tissue and increase energy dissipation in obese mice.
