First Hospital of Changsha 1 article published in JoVE Cancer Research Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue Guang Lei*1, Haiyan Yang*1, Ting Hong2, Xiangyu Zhang1, Nong Yang1, Yongchang Zhang1 1Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 2Hunan ClinicaI Research Center in Gynecologic Cancer, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University Here, we present a protocol of elastic staining to identify elastic fibers in pT3N0M0 gastric cancer tissues on formalin-fixed, paraffin-embedded sections. Subsequently, we describe a method to determine whether tumor cells invade beyond the elastic lamina.
