Huacells Corp 1 article published in JoVE Bioengineering A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level Fenfang Li1, Fang Yuan2, Georgy Sankin1, Chen Yang1, Pei Zhong1 1Mechanical Engineering and Materials Science, Duke University, 2Huacells Corp A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.