The First Hospital of Jilin University 3 articles published in JoVE Medicine Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice Ping Ren1,2,3, Chunyan Yang2,4, Laren A. Lofchy2, Guankui Wang2, Fangfang Chen*2,4,5, Dmitri Simberg*2 1Department of Thoracic Surgery, The First Hospital of Jilin University, 2The Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, 3Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, 4Department of Cardiology, China-Japan Union Hospital of Jilin University, 5Department of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University A protocol is described for in situ perfusion of the mouse lower body, including the bladder, the prostate, sex organs, bone, muscle and foot skin. Immunology and Infection Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay Caiyun Liu1, Mingqin Zhu1, Ying Wang1 1Department of Neurology and Neuroscience Center, The First Hospital of Jilin University Cell-based assay is a widely used method to detect serum anti-aquaporin-4 immunoglobulin G. This method could be applied to clinical diagnosis and scientific researches of neuromyelitis optical spectrum disorders. Bioengineering A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation Yang Bai1,2, Enoch Yeung2, Cecillia Lui2, Chin Siang Ong2, Isaree Pitaktong2, Chenyu Huang2, Takahiro Inoue2, Hiroshi Matsushita2, Chunye Ma2, Narutoshi Hibino2 1Department of Cardiac Surgery, The First Hospital of Jilin University, 2Division of Cardiac Surgery, Johns Hopkins Hospital This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior.
