Northwestern Polytechnical University 3 articles published in JoVE Immunology and Infection Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226 Yang Xie*1, Yuan Zhang*2, Yitian Liu3, Yuling Wang4, Kun Cheng4, Ran Zhuang4, Ka Bian1 1Otolaryngological Department of Tangdu Hospital, Fourth Military Medical University, 2Institute of Medical Research, Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital, Fourth Military Medical University, 4Department of Immunology, Fourth Military Medical University Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated. Medicine Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug Weijia Lu*1, Ruimin Miao*1, Sijun Hu1, Junhong Liu1, Fanqi Jin1, Rui Xue Zhang1 1Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress. Medicine Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch Yaguang Li1, Junhong Liu2,3, Gaixia Liu1, Zhenhong Pan2,3, Mingxia Zhang1, Yao Ma4, Qingxia Wei1,5, Hongping Xia1,6, Rui Xue Zhang1,2, Junjun She1 1 The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.