Shandong First Medical University & Shandong Academy of Medical Sciences 1 article published in JoVE Medicine Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography Di Liu*1, Xizhu Xu*2, Yuejin Li2, Jie Zhang1, Xiaoyu Zhang1, Qihuan Li1, Haifeng Hou2, Dong Li2, Wei Wang1,2,3, Youxin Wang1 1Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, 2School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, 3School of Medical and Health Sciences, Edith Cowan University Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.
