Shanghai Jiao Tong University 9 articles published in JoVE Medicine Establishing a Device for Sleep Deprivation in Mice Jianqing Chen*1, Jingze Wei*1, Xiaoying Ying*2, Fan Yang*2, Yichao Zhao*2, Jun Pu1,2 1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University The present protocol outlines a method for setting up a cost-effective rocker platform-based device used for inducing sleep deprivation in mice. This device has proven to be effective in causing disruptions in electroencephalogram (EEG)-evidenced sleep patterns, as well as inducing metabolic and molecular changes associated with sleep deprivation. Biology A 3-D Visualization Technique for Bone Remodeling in a Suture Expansion Mouse Model Ziduan Ding1,2, Ruomei Li1,2, Yufeng Duan1,2, Zhenxia Li1,2, Bing Fang1,2, Dian Jing1,2 1Department of Orthodontics, China Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, 2National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University This protocol presents a standardized suture expansion mouse model and a 3-D visualization method to study the mechanobiological changes of the suture and bone remodeling under tensile force loading. Medicine Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement Yuanqi Liu*1,2,3,4,5, Siyuan Sun*1,2,3,4,5, Ziyi Jiang*6, Xinyi Gong1,2,3,4,5, Yiling Yang1,2,3,4,5, Yanfei Zhu1,2,3,4,5, Hongyuan Xu1,2,3,4,5, Anting Jin1,2,3,4,5, Xiangru Huang1,2,3,4,5, Xin Gao1,2,3,4,5, Tingwei Lu1,2,3,4,5, Jingyi Liu1,2,3,4,5, Xinyu Wang1,2,3,4,5, Qinggang Dai2,3,4,5,7, Lingyong Jiang1,2,3,4,5 1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Stomatology, Shanghai Jiao Tong University, 3National Center for Stomatology, 4National Clinical Research Center for Oral Diseases, 5Shanghai Key Laboratory of Stomatology, 6Shanghai Starriver Bilingual School, 7The 2nd Dental Center, Ninth People’s Hospital, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine This study provides a protocol for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describes methods for analyzing alveolar bone remodeling during orthodontic tooth movement, thus shedding light on skeletal mechanical biology. Medicine Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model Sijuan Sun*1, Hongbin Zhu*2, Shoubao Wang3, Xiuxia Xu4, Lincai Ye2,5,6 1Department of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, 2Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiaotong University, 3Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 4Department of Radiology, Huangpu Branch, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 5Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, 6Shanghai Institute for Pediatric Congenital Heart Disease, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University This protocol presents the establishment and confirmation of a postnatal right ventricular volume overload (VO) model in mice with abdominal arteriovenous fistula (AVF), which can be applied to investigate how VO contributes to postnatal heart development. Biology Isolation and Transcriptome Analysis of Plant Cell Types Jun Zhang1, Mayra Ahmad1, Rongrong Xie1, Hongbo Gao1 1Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University The feasibility and effectiveness of high-throughput scRNA-seq methods herald a single-cell era in plant research. Presented here is a robust and complete procedure for isolating specific Arabidopsis thaliana root cell types and subsequent transcriptome library construction and analysis. Bioengineering Viscoelastic Characterization of Soft Tissue-Mimicking Gelatin Phantoms using Indentation and Magnetic Resonance Elastography Yuan Feng1, Suhao Qiu1, Yu Chen1, Runke Wang1, Zhao He1, Linghan Kong1, Yi Chen1, Shengyuan Ma1 1School of Biomedical Engineering, Shanghai Jiao Tong University This article presents a demonstration and summary of protocols of making gelatin phantoms that mimic soft tissues, and the corresponding viscoelastic characterization using indentation and magnetic resonance elastography. Biology Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion Chao E*1, Liqiang Dai*1,2, Jiaqi Tian3,4, Lin-Tai Da4, Jin Yu5,6,7 1Beijing Computational Science Research Center, 2Shenzhen JL Computational Science and Applied Research Institute, 3School of Medical Informatics and Engineering, Xuzhou Medical University, 4Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 5Department of Physics and Astronomy, University of California, Irvine, 6Department of Chemistry, University of California, Irvine, 7NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine The goal of this protocol is to reveal structural dynamics of one-dimensional diffusion of protein along DNA, using a plant transcription factor WRKY domain protein as an exemplary system. To do this, both atomistic and coarse-grained molecular dynamics simulations along with extensive computational samplings have been implemented. Medicine A Modified Simple Method for Induction of Myocardial Infarction in Mice Chenglin Jiang*1,2, Jianqing Chen*1,2, Yichao Zhao*2, Dasheng Gao1, Hongju Wang1, Jun Pu1,2 1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University Under adequate anesthesia, the mouse heart was externalized through the intercostal space, and myocardial infarction was successfully induced by ligating the left anterior descending artery (LAD) using materials readily available in most laboratories. Developmental Biology Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue Yiding Qi*1, Xiang Miao*2, Lian Xu3, Mengxia Fu3, Shi Peng4, Kailei Shi5, Jun Li4, Maoqing Ye5, Ruogu Li1 1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre+/-;Rosa26RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.
