Army Medical University 6 articles published in JoVE Medicine Microscopic Replantation of Penile Glans Amputation Due to Circumcision Da-Chun Jin*1, Bo Zhou*1, Jun Li2, Cong-Cong Bao1, Yong Luo1, Yong Zhang1, Peng Wang1, Gang Bi1, Yan-Feng Li1 1Department of Urology, Daping Hospital, Army Medical University, 2 The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision. Medicine A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model Xin Mao*1, Peng Xian*1, Hang You1,2, Gang Huang3, Jun Li1 1Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 2School of Medicine, Chongqing University, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique. Medicine A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection Hang You1,2, Xin Mao2, Chenyang Wang2, Gang Huang3, Marcus Groettrup4,5, Jun Li2 1School of Medicine, Chongqing University, 2Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, 4Division of Immunology, Department of Biology, University of Konstanz, 5Biotechnology Institute Thurgau, University of Konstanz The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis. Neuroscience A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid Ling-Yi Liao1,2,3, Ying-Qian Zhang4, Benson Wui-Man Lau5, Qiong Wu6, Zi-Yan Fan1,3, Qiang Gao1,3, Zhi-Hui Zhong6,7 1Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 2Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, 3Key Laboratory of Rehabilitation Medicine in Sichuan Province, West China Hospital, Sichuan University, 4Department of physiology, Southwest Medical University, 5Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, 6Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 7Sichuan Kangcheng Biotech Co., Inc. Here, we describe the procedure for a pilot study to explore the effect of repetitive transcranial magnetic stimulation with different frequencies (1 Hz/20 Hz/40 Hz) on Aβ and tau metabolism in rhesus monkey cerebrospinal fluid. Cancer Research Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice Lisha Wang1, Zhiming Wang1, Junyi Guo2, Huayu Lin1, Shuqiong Wen2, Qiao Liu1, Yiding Li3, Qing Wu1, Leiqiong Gao1, Xiangyu Chen1, Luoyingzi Xie1, Qin Tian1, Jianfang Tang1, Zhirong Li1, Li Hu1, Juan Wang4, Lifan Xu1, Qizhao Huang5, Lilin Ye1 1Institute of Immunology, Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital, Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, 5Cancer Center, The General Hospital of Western Theater Command Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses. Immunology and Infection Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis Eunbee Park1, Megan A. Evans4, Heather Doviak4, Keita Horitani4, Hayato Ogawa4, Yoshimitsu Yura4, Ying Wang2, Soichi Sano3,4, Kenneth Walsh1,4 1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, 2Department of Cardiology, Xinqiao Hospital, Army Medical University, 3Department of Cardiology, Osaka City University Graduate School of Medicine, 4Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models.
