Westlake Laboratory of Life Sciences and Biomedicine 1 article published in JoVE Neuroscience Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice YangDong Wang1,2,3,4, DanYang Zhu5, BaoYue Liu1,2,3,6, Kiryl D. Piatkevich1,2,3 1School of Life Sciences, Westlake University, 2Westlake Laboratory of Life Sciences and Biomedicine, 3Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, 4 This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice.